Zero Chrom® Platform
Industry-leading column-free plasmid manufacturing.
FDA & NMPA approved · More than 95% supercoiled · Faster purification · Scalable production · Cost reduction of up to 60–70%.
Platform advantages
A single-use, chromatography-free plasmid platform
Faster purification
No column chromatography is required.
Low regulatory risk
Single-use systems help reduce contamination risk.
High-quality plasmid
More than 95% supercoiled plasmid.
FDA & NMPA approved
Zero Chrom® plasmids have supported IND approvals.
High throughput
More than 300 batches per year.
Scalable production
From 1L to 30L for research and GMP-grade plasmids.
Off-the-shelf
Available plasmids
Zero Chrom® plasmids are available off the shelf in both process development and GMP grades — from milligram to gram scale.
Off-the-shelf
AAV helper & Rep-CapX plasmids
Off-the-shelf
LVV helper & packaging plasmids
Off-the-shelf
GFP plasmids
Off-the-shelf
Luciferase plasmids
Process
Upstream & downstream — end to end
Upstream
5 production lines · 350+ batches / year.
- E. coli plasmid production
- Clone selection & PCB banking
- Fermentation optimization
- R&D fermentation 1–10L · 5mg–2g
- GMP fermentation 6L · 1–5g
- GMP fermentation 30L · 5–25g
Downstream
High yield, high purity, low residuals — without chromatography. Optional AEX or HIC available when required.
50–70%
Overall yield
>95%
Supercoiled
<10 EU/mg
Endotoxin
3–15 g
30L post-purification
Applications
Plasmids that power advanced therapies
Viral vector production
Packaging plasmids for AAV, LVV and other viral vectors used in cell & gene therapy programs.
mRNA in vitro transcription
High-quality DNA templates for IVT — mRNA vaccines and therapeutics.
Direct plasmid therapeutics
DNA-based vaccines and plasmid vector applications administered directly.